研究業績

論文

  • In Vitro Assembly and Cellulolytic Activity of a Beta-Glucosidase-Integrated Cellulosome Complex.
    Hirano, K., Saito, T., Shinoda, S., Haruki, M., and Hirano, N.
    FEMS Microbiol. Lett. (2019) 366, fnz209.
  • Comparative Biochemical Analysis of Cellulosomes Isolated from Clostridium clariflavum DSM 19732 and Clostridium thermocellum ATCC 27405 Grown on Plant Biomass.
    Shinoda, S., Kurosaki, M., Kokuzawa, T., Hirano, K., Takano, H., Ueda, K., Haruki, M., and Hirano, N.
    Appl. Biochem. Biotechnol. (2019) 187, 994-1010.
  • Alkyne-Functionalized Cationic Polysiloxane Polymers Conjugated with Targeting Molecules by Click Reactions for Targeted DNA Delivery.
    Kihara, Y., Maeda, R., Imaizumi, A., Ichikawa, T., Nemoto, N., Ishihara, T., Hirano, N., and Haruki, M.
    J. Nanosci. Nanotech. (2017) 17, 5081-5089.
  • Enzymatic Diversity of the Clostridium thermocellum Cellulosome Is Crucial for the Degradation of Crystalline Cellulose and Plant Biomass.
    Hirano, K., Kurosaki, M., Nihei, S., Hasegawa, H., Shinoda, S., Haruki, M., and Hirano, N.
    Sci. Rep. (2016) 6, 35709.
  • Stoichiometric Assembly of the Cellulosome Generates Maximum Synergy for the Degradation of Crystalline Cellulose, as Revealed by In Vitro Reconstitution of the Clostridium thermocellum Cellulosome.
    Hirano, K., Nihei, S., Hasegawa, H., Haruki, M., and Hirano, N.
    Appl. Environ. Microbiol. (2015) 81, 4756-4766.
    "Spotlight" of the Volume 81, Issue 14
    "Articles of Significant Interest Selected from This Issue by the Editors"
  • Protein Stabilization Utilizing a Redefined Codon.
    Ohtake, K., Yamaguchi, A., Mukai, T., Kashimura, H., Hirano, N., Haruki, M., Kohashi, S., Yamagishi, K., Murayama, K., Tomabechi, Y., Itagaki, T., Akasaka, R., Kawazoe, M., Takemoto C., Shirouzu, M., Yokoyama, S., and Sakamoto, K.
    Sci. Rep. (2015) 5, 9762.
  • Synthesis of Alkyne-Functionalized Amphiphilic Polysiloxane Polymers and Formation of Nanoemulsions Conjugated with Bioactive Molecules by Click Reactions.
    Kihara, Y., Ichikawa, T., Nemoto, N., Ishihara, T., Hirano, N., and Haruki, M.
    Polymer J. (2014) 46, 175-183.
  • Cell-Free Protein Synthesis and Substrate Specificity of Full-Length Endoglucanase CelJ (Cel9D-Cel44A), the Largest Multi-Enzyme Subunit of the Clostridium thermocellum Cellulosome.
    Hirano, N., Hasegawa, H., Nihei, S., and Haruki, M.
    FEMS Microbiol. Lett.(2013) 344, 25-30.
  • TG1 Integrase-Based System for Site-Specific Gene Integration into Bacterial Genomes.
    Muroi, T., Kokuzawa, T., Kihara, Y., Kobayashi, R., Hirano, N., Takahashi, H., and Haruki, M.
    Appl. Microbiol. Biotechnol. (2013) 97, 4039-4048.
  • Enhancement of the Enzymatic Activity of Escherichia coli Acetyl Esterase by a Double Mutation Obtained from Random Mutagenesis.
    Kobayashi, R., Hirano, N., Kanaya, S., and Haruki, M.
    Biosci. Biotechnol. Biochem. (2012) 76, 2082-2088.
  • Site-Specific Genome Integration in Alphaproteobacteria Mediated by TG1 Integrase.
    Morita, K., Morimura, K., Fusada, N., Komatsu, M., Ikeda, H., Hirano, N., and Takahashi, H.
    Appl. Microbiol. Biotechnol. (2012) 93, 295-304.
  • Site-Specific Recombinases as Tools for Heterologous Gene Integration.
    Hirano, N., Muroi, T., Takahashi, H., and Haruki, M.
    Appl. Microbiol. Biotechnol. (2011) 92, 227-239.
    "Invited Review"
  • Site-Specific Recombination System Based on Actinophage TG1 Integrase for Gene Integration into Bacterial Genomes.
    Hirano, N., Muroi, T., Kihara, Y., Kobayashi, R., Takahashi, H., and Haruki, M.
    Appl. Microbiol. Biotechnol. (2011) 89, 1877-1884.
  • Enhancement of the Enzymatic Activity of Escherichia coliAcetyl Esterase by Random Mutagenesis.
    Kobayashi, R., Hirano, N., Kanaya, S., Saito, I., and Haruki, M.
    J. Mol. Catalysis B: Enzymatic (2010) 67, 155-161.
  • In Vitro Characterization of the Site-Specific Recombination System Based on Actinophage TG1 Integrase.
    Morita, K., Yamamoto, T., Fusada, N., Komatsu, M., Ikeda, H., Hirano, N., and Takahashi, H.
    Mol. Genet. Genomics (2009) 282, 607-616.
  • The Site-Specific Recombination System of Actinophage TG1.
    Morita, K., Yamamoto, T., Fusada, N., Komatsu, M., Ikeda, H., Hirano, N., and Takahashi, H.
    FEMSMicrobiol. Lett. (2009) 297, 234-240.
  • The Ser176 of T4 Endonuclease IV Is Crucial for the Restricted and Polarized dC-Specific Cleavage of Single-Stranded DNA Implicated in Restriction of dC-Containing DNA in Host Escherichia coli.
    Hirano, N., Ohshima, H., Sakashita, H., and Takahashi, H.
    Nucleic Acids Res. (2007) 35, 6692-6700.
  • A Hexanucleotide Sequence (dC1-dC6 Tract) Restricts the dC-Specific Cleavage of Single-Stranded DNA by Endonuclease IV of Bacteriophage T4.
    Ohshima, H., Hirano, N., and Takahashi, H.
    Nucleic Acids Res. (2007) 35, 6681-6689.
  • Biochemical Analysis of the Substrate Specificity and Sequence Preference of Endonuclease IV from Bacteriophage T4, a dC-Specific Endonuclease Implicated in Restriction of dC-Substituted T4 DNA Synthesis.
    Hirano, N., Ohshima, H., and Takahashi, H.
    Nucleic Acids Res. (2006) 34, 4743-4751.
  • Tolerance for Random Recombination of Domains in Prokaryotic and Eukaryotic Translation Systems: Limited Inter-Domain Misfolding in a Eukaryotic Translation System.
    Hirano, N., Sawasaki, T., Tozawa, Y., Endo, Y., and Takai, K.
    Proteins (2006) 64, 343-354.
  • Efficient Cleavage of RNA at High Temperatures by a Thermostable DNA-Linked Ribonuclease H.
    Haruki, M., Nogawa, T., Hirano, N., Chon, H., Tsunaka, Y., Morikawa, M., and Kanaya, S.
    Protein Eng. (2000) 13, 881-886.
  • Enhancement of the Enzymatic Activity of Ribonuclease HI from Thermus thermophilus HB8 with a Suppressor Mutation Method.
    Hirano, N., Haruki, M., Morikawa, M., and Kanaya, S.
    Biochemistry (2000) 39, 13285-13294.
  • Stabilization of Ribonuclease HI from Thermus thermophilusHB8 by the Spontaneous Formation of an Intramolecular Disulfide Bond.
    Hirano, N., Haruki, M., Morikawa, M., and Kanaya, S.
    Biochemistry (1998) 37, 12640-12648.

著書

  • T4 denB Gene Product, an Endonuclease (Endo IV) Targeting at the Achilles’ Tendon of DNA Replication.
    Hirano, N., Ohshima, H., and Takahashi, H.
    - Adaptive Gene Regulations – from Microorganisms to Organelles -
    Fujiwara, M., Tanaka, K., and Takahashi, H. (Ed.)
    Research Signpost, Kerala, India. (2008) 135-148.

解説・総説

  • 植物バイオマスからの有用物質生産に向けた取り組み
    平野 展孝
    財界ふくしま (2019) 第48巻 第5号 119-125.
  • 駕籠に乗る人、担ぐ人、そのまた草鞋を作る人
    平野 展孝
    生物工学会誌 (2014) 第92巻 第6号 305.
  • 分岐点移動・ホリデイ接合解離酵素
    平野 展孝
    生化学 (2013) 第85巻 第11号 1039.
  • インテグラーゼ・トランスポソーム
    平野 展孝
    生化学 (2013) 第85巻 第5号 372.
  • ゲノム工学ツールとしてのセリンタイプ・インテグラーゼ
    平野 展孝, 春木 満
    生化学 (2013) 第85巻 第5号 345-349.
  • 草質・木質(リグノセルロース)系バイオマスからのバイオエタノール生産における現状と課題
    平野 展孝
    福島の進路 (2011) No.342, 27-30.
  • 原核、真核生物翻訳系における蛋白質フォールディングの比較
    平野 展孝
    生産と技術 (2007) 第59巻 第4号 59-61.

招待・依頼講演

  • 平成26年度 化学系学協会東北大会 生体分子セッション (2014)
    植物バイオマス分解酵素複合体の再構成
    平野 展孝
  • 平成22年度 化学系学協会東北大会 若手シンポジウム (2010)
    無細胞蛋白質合成系を用いた蛋白質・酵素の研究
    平野 展孝

受賞

  • 第1回 新化学技術研究奨励賞 (2012)
    植物性バイオマス分解酵素複合体の合成生物学
    平野 展孝
  • 第9回 酵素応用シンポジウム研究奨励賞 (2008)
    バクテリオファージが生産する細胞毒性酵素の無細胞合成と機能解析
    平野 展孝

報道発表

  • 福島民友新聞 (2015)
    環境、健康 活用図る
  • 日経産業新聞 (2012)
    植物成分、糖に効率分解